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实现小麦无麸的遗传工程技术

核心提示:一个国际研究组成功通过遗传工程,实现小麦无麸。研究人员的工作重点在于DEMETER (DME),一种能诱导产生小麦麸质的基因的酶。利用遗传工程技术,研究者成功抑制了85.6%的DME,从而减少了小麦种子76.4%的麸质。

  【中国生物产业网www.cnbioindustry.cn】2013年1月6日讯  一个国际研究组成功通过遗传工程,实现小麦无麸。研究人员的工作重点在于DEMETER (DME),一种能诱导产生小麦麸质的基因的酶。利用遗传工程技术,研究者成功抑制了85.6%的DME,从而减少了小麦种子76.4%的麸质。

  研究组专家来自中国、德国和美国。他们声称,这种经过处理的小麦种子制成的面粉适合制作面包,下一步他们的研究工作将确认这种谷粒是否能够适用于患乳糜泻人群食用。

  Abstract:

  Wheat supplies about 20% of the total food calories consumed worldwide and is a national staple in many countries. Besides being a key source of plant proteins, it is also a major cause of many diet-induced health issues, especially celiac disease. The only effective treatment for this disease is a total gluten-free diet.

  The present report describes an effort to develop a natural dietary therapy for this disorder by transcriptional suppression of wheat DEMETER (DME) homeologs using RNA interference. DME encodes a 5-methylcytosine DNA glycosylase responsible for transcriptional derepression of gliadins and low-molecular-weight glutenins (LMWgs) by active demethylation of their promoters in the wheat endosperm.

  Previous research has demonstrated these proteins to be the major source of immunogenic epitopes. In this research, barley and wheat DME genes were cloned and localized on the syntenous chromosomes. Nucleotide diversity among DME homeologs was studied and used for their virtual transcript profiling.

  Functional conservation of DME enzyme was confirmed by comparing the motif and domain structure within and across the plant kingdom. Presence and absence of CpG islands in prolamin gene sequences was studied as a hallmark of hypo- and hypermethylation, respectively.

  Finally the epigenetic influence of DME silencing on accumulation of LMWgs and gliadins was studied using 20 transformants expressing hairpin RNA in their endosperm. These transformants showed up to 85.6% suppression in DME transcript abundance and up to 76.4% reduction in the amount of immunogenic prolamins, demonstrating the possibility of developing wheat varieties compatible for the celiac patients.

  更多生物工程生物医药生物产业资讯,尽在中国生物产业网

  编辑:Cassie.D

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